Quantitation Of Protein

Introduction In today’s world we search on pharmaceutical drugs more than ever and production of these drugs same(p) insulin or human growth hormone, require quantitation of protein. By do experiments on quantitation of protein we give the axe discover windy methods or new shipway to achieve this and as well as see which method is the most cost-effective. all told company wants an proceeds over the former(a) companies, so if they stop find a faster or more efficient way of quantitating protein, they can absorb more money compargond to the other companies. This lab uses a guess of spectrophotometry and an understanding of the Beer-Lambert law; it also uses three hindrances utilise to quantitate protein. Spectrophotometry uses a device called a Spectrophotometer which can measure the absorbance of unused at different wavelengths in a resoluteness. The Beer-Lambert’s law relates the absorbance to the submerging of the solution, the pa thlength of the cuvette and the extinction coefficient (A=ecl). Three stresss used to quantitate protein in this lab are Coomassie Blue, Lowry and UV direct. Coomassie blue is a dye binding method, where it binds to the protein make up in the solution and changes the discolour of the solution which is then thrifty by the spectrophotometer. Lowry assay is where the cupric ions react with the peptide bonds under alkalic conditions ever-changing the colour of the solution which can then be measured. The UV direct assay measures the amount of UV light absorbed by the solution. The documental of this lab is to find concentrations of proteins utilise standards that were made for each assay; hoping to see similar concentrations for all proteins. Methods (Carleton University, 2009) Results In figure 1 you can see the absorbance set across different wavelengths for the p-np solution. The measurable thing to note is at what wavelength the graph peaks at. Table 1: Absorba nce and Extinction coefficient values for ch! anging concentrations of...If you want to get a full essay, order it on our website: BestEssayCheap.com

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